Part:BBa_K2000007:Design

hKLF4-active

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 265
  

Design Notes

we have to mutant a Pst I site at 468 site.

Source

atgaggcagccacctggcgagtctgacatggctgtcagcgacgcgctgctcccgtccttctccacgttcgcgtccggcccggcgggaagggagaagacactgcgtccagcaggtgccccgaataaccgctggcgggaggagctctcccacatgaagcgacttcccccggtgcttcccggccgcccctacgacctggcggcggcgaccgtggccaccgacctggagagtggaggagtcggcgcggcttgcggcagcagcaacccggctcttctgccccggagggagacggaggagttcaatgatctcctggacctggactttatcctctccaactcgctgtcccatcaggagtccgtggccgccacggtgtcctcctcggcatcagcctcatcctcgtcctccccgtcgagcagcggtccagccagtgcgccctccacctgccgcttcagctatccaatccgggccggggg